This paper presents the results of a dynamic response evaluation of a segmental bridge during two construction stages: before connecting the final segment of the bridge and after connecting the final segment of the bridge but prior to opening the bridge to traffic. The vibration signals obtained from Ambient Vibration Testing (AVT) campaigns were processed in order to obtain the modal parameters of the bridge during the two construction stages. Modal parameters experimentally obtained for the first stage were compared with those obtained from Finite Element (FE) models considering different construction loads scenarios. Finally, modal parameters experimentally obtained for the second stage were used to update its corresponding FE model considering two scenarios, before and after the installation of the asphalt pavement. The results presented in this paper demonstrated that a rigorous construction control is needed in order to effectively calibrate FE models during the construction process of segmental bridges.